Background:

This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This complex plays a role in the DNA damage response, where it is responsible for the stable accumulation of BRCA1 at DNA break sites. The component encoded by this gene can specifically cleave Lys 63-linked polyubiquitin chains, and it regulates the abundance of these polyubiquitin chains in chromatin. The loss of this gene results in abnormal angiogenesis and is associated with syndromic moyamoya, a cerebrovascular angiopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 5.

Applications:

ELISA, WB

Name of antibody:

BRCC3

Immunogen:

Fusion protein of human BRCC3

Full name:

BRCA1/BRCA2-containing complex subunit 3

Synonyms:

C6.1A; BRCC36; CXorf53

SwissProt:

P46736

ELISA Recommended dilution:

5000-10000

WB Predicted band size:

36 kDa

WB Positive control:

Human heart tissue and A549 cell lysates

WB Recommended dilution:

200-1000